In order to evaluate if vaccination changes responses


In order to evaluate if vaccination changes responses

of the anti-malaria effectors spleen and liver to blood stage malaria, BALB/c mice succumbing to infection with Plasmodium chabaudi were compared to those surviving after vaccination.

Methods: selleck inhibitor Mice were vaccinated with host cell plasma membranes isolated from P. chabaudi-infected erythrocytes. Hepatic and splenic capacity to trap particulate material was determined after injection of fluorescent polystyrol beads. Hepatic gene expression was measured using real-time RT-PCR and Northern blotting.

Results: Survival of BALB/c mice was raised from 0% to 80% and peak parasitaemia was decreased by about 30% by vaccination. Vaccination boosted particle trapping capacity of the liver during crisis when splenic trapping selleck products is minimal due to spleen ‘closing’. It also attenuated malaria-induced inflammation, thus diminishing severe damages and hence liver failure. Vaccination increased hepatic IFN-gamma production but mitigated acute phase response. Vaccination has a complex influence on infection-induced changes in expression of hepatic nuclear

receptors (CAR, FXR, RXR, and PXR) and of the metabolic enzymes Sult2a and Cyp7a1. Although vaccination decreased CAR mRNA levels and prevented Cyp7a1 suppression by the CAR ligand 1,2-bis [2-(3,5dichloropyridyloxy)] benzene (TCPOBOP) on day 8 p.i., Sult2a-induction by TCPOBOP was restored.

Conclusion: These data support the view that the liver is an essential effector site for a vaccine against blood stage malaria: vaccination attenuates malaria-induced inflammation thus improving hepatic metabolic activity and particle trapping activity of the liver.”
“The prevalence of coronary pathologic findings in patients with left ventricular hypertrabeculation/noncompaction (LVHT)

is unknown. The study in a cohort of consecutive LVHT patients aimed to assess how often coronary angiography (CA) had been performed, if clinical findings and prognosis differed between patients with and without CA, how often pathologic findings of the coronary arteries were found, and if there were differences between LVHT patients with and without coronary pathologic findings.

Between 1995-2007 LVHT was diagnosed in 113 patients (mean age 53 years, 29% females, 67% neuromuscular disorders). CA Selleck JQ1 had been performed in 52. Patients with CA had more exertional dyspnoea (79 vs. 61%, p < 0.05), angina pectoris (42 vs. 10%, p < 0.001), hypertension (44 vs. 25%, p < 0.05) and larger left ventricles (66 vs. 60 mm, p < 0.001) than patients without. No anomalously originating coronary arteries were found. Patients with coronary arteriosclerosis (n = 8) had more right-bundle-branch block than patients without (25 vs. 0%, p < 0.05). Mortality was 5.8% per year and did neither differ between patients with and without CA nor with and without coronary arteriosclerosis.

Coronary arteriosclerosis is rarely associated with LVHT and does not seem to affect prognosis.

17 mu m, 100, and 219 nm, respectively Amino acids like glycine,

17 mu m, 100, and 219 nm, respectively. Amino acids like glycine, proline, and hydroxyproline were found with highest level in all the body parts of

the extracted gelatin using HPLC.”
“Recently, solid state photovoltaic Schottky diodes, able to detect ionizing radiation, in particular, x-ray and ultraviolet radiation, have been developed at the University of Rome “”Tor Vergata.”" We report 5-Fluoracil order on a physical and electrical properties analysis of the device and a detailed study of its detection capabilities as determined by its electrical properties. The design of the device is based on a metal/nominally intrinsic/p-type diamond layered structure obtained by microwave plasma chemical vapor deposition of Selleckchem CP 868596 homoepitaxial single crystal diamond followed by thermal evaporation of a metallic contact. The device can operate in an unbiased mode by using the built-in potential arising from the electrode-diamond junction. We compare the expected response of the device to photons of various energies calculated through Monte Carlo simulation

with experimental data collected in a well controlled experimental setup i.e., monochromatic high flux x-ray beams from 6 to 20 keV, available at the Diamond Light Source synchrotron in Harwell (U.K.).”
“Hybrid PTFE/Kevlar fabric was surface-modified by air plasma to improve the adhesion to phenolic resin. The tribological properties of fabric composite with or without plasma-treated 4EGI-1 hybrid PTFE/Kevlar fabric were investigated in detail. Friction and wear tests showed that friction-reducing

and antiwear ability of hybrid PTFE/Kevlar fabric composite were remarkably improved after plasma treatment. Scanning electron microscopy and X-ray photoelectron spectroscopy results indicated that the fiber surfaces were etched and the contents of oxygen and nitrogen increased after plasma treatment, which was favorable for improved fiber/resin bonding strength. The enhancement contributed to the improved tribological properties of hybrid PTFE/Kevlar fabric composite after plasma treatment. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 114: 3980-3986, 2009″
“Background: Clinical data and data on outcome of extra-osseous Ewing tumors are scarce. Procedure: After a search for Ewing tumors in the database of a single institution over a period of 20 years, 16 out of 192 cases were found to have extra-osseous primary tumors. Results: Ages at initial diagnosis ranged from 2.5 to 17 years. Follow-up period ranged from 4 months to 24.8 years (mean 8.4 years). Eleven patients were treated according to protocols for Ewing tumors, while in 4 cases soft tissue protocols were used. In a single patient only surgery was done. Two patients had progressive disease despite chemotherapy; a third patient had only tumor response on the initial 2 chemotherapy courses. All 3 patients with initially metastatic disease died. One patient developed a second malignancy.

Thus, as a result of obesity, mobility and quality of life are af

Thus, as a result of obesity, mobility and quality of life are affected, and this is in part due to quantitative and qualitative impairments in skeletal muscle. In addition, the insulin resistance related to obesity results not only in defective insulin-stimulated glucose disposal but has also detrimental consequences

on protein metabolism at the skeletal muscle level and whole-body level. This review highlights the involvement click here of fat accumulation and insulin resistance in metabolic disorders occurring in skeletal muscle during the development of obesity, and the impairments in the regulation of protein metabolism and protein turnover in the links between obesity, metabolic inflammation and insulin resistance.”
“Early diagnosis of osteoporosis and estimation of subjects that are at high risk for fracture, is neccesary for osteoporosis treatment. Dual-energy X-ray absorptometry

(DXA) is a modern method for bone mineral density (BMD) evaluation. However, along BMD, clinical risk factors may significantly influence fracture development. Therefore, FRAX algorithm was designed for the assessment of a ten-year risk for serious osteoporotic fractures (SOF), as well as hip fractures. In the current study, we tried to evaluate the possible lumbal spine and hip BMD influence on ten year risk for SOF and hip fractures and potential role of FRAX in predicting the therapy in postmenopausal women with osteopenia. We performed the selleck chemical Ispinesib solubility dmso study on 385 postmenopausal women. According to the DXA measurements, at the lumbal (L) spine (L1-L4) and hip (femor neck), patients were then classified as normal, osteopenic, or osteoporotic. BMD evaluation included the L spine and the hip (subgroup 1), and only on the L spine (subgroup 2). By filling up the FRAX questionnaire, a ten-year risk for SOF

fracture and hip fracture was calculated. BMD evaluation, in complete patient’s group and in subgroup 1, resulted in the highest number of osteoporosis (61.04%, 48.08%, retrospectively), while ospeopenia was a main finding in subgroup 2. In the subgroup 1, a high risk for SOF and hip fracture was detected in 16.45% and with high risk for hip fracture in 11.38% subjects. In subgroup 2, only high risk for hip fracture was observed in 3.16% subjects, indicating the active medicament treatment. Simultaneously, correlation of BMD results with FRAX values for SOF and hip fracture, showed significant negative correlation (p < 0.001). Obtained results showed significant role of femur neck BMD evaluation in predicting the future factors, which may, together with FRAX analysis, improve the therapy approach in postmenopausal women with ospeopenia.”
“Factors as age, sex, smoking, duration of leg pain, working status, type/level of disc herniation and psychosocial factors have been demonstrated to be of importance for short-term results after lumbar discectomy. There are few studies with long-term follow-up.

Ten healthy adult volunteers (mean age +/- standard deviation, 25

Ten healthy adult volunteers (mean age +/- standard deviation, 25 years +/- 4; seven men, three women) underwent navigator-gated three-dimensional MR angiography of the RCA at 7 T and 3 T. For 7 T, a custom-built quadrature radiofrequency transmit-receive surface coil was used. At 3 T, a commercial body radiofrequency

transmit coil and a cardiac coil array for signal reception were used. Segmented k-space gradient-echo imaging with spectrally selective adiabatic fat suppression was performed, and imaging parameters were similar at both field strengths. Contrast-to-noise ratio between blood and epicardial fat; signal-to-noise ratio of the blood pool; RCA vessel sharpness, diameter, and length; and navigator efficiency were quantified at both field strengths and compared by using a Mann-Whitney U test.

Results: AP24534 The contrast-to-noise ratio between blood and epicardial fat was

significantly improved at 7 T when compared with that at 3 T (87 +/- 34 versus 52 +/- 13; P = .01). Signal-to-noise ratio of the blood pool was increased at 7 T (109 6 47 versus 67 +/- 19; P = .02). Vessel sharpness obtained at 7 T was also higher (58% +/- 9 versus 50% +/- Selleckchem LCL161 5; P = .04). At the same time, RCA vessel diameter and length and navigator efficiency showed no significant field strength-dependent difference.

Conclusion: In our quantitative and qualitative study comparing in vivo human imaging of the RCA at 7 T and 3 T in young healthy volunteers, parameters related to image quality attained at 7 T equal or surpass those from 3 T. (C) RSNA, 2010″
“Relaxed epitaxial 100(pc) and 111(pc) oriented films (350 Selleck MK-8931 nm) of Pb(Zr(1-x)Ti(x))O(3) (0.2 <= x <= 0.4) on SrRuO(3)/SrTiO(3) substrates were grown by pulsed laser deposition and

studied using high resolution synchrotron X-ray diffraction and transmission electron microscopy. The dielectric behavior and ferroelectric phase transition temperatures of the films were consistent with bulk PZT. However, weak 1/2311(pc) reflections in x-ray diffraction profiles were recorded above bulk T(Tilt) (as indicated in the Jaffe, Cooke, and Jaffe phase diagram, where pc denotes pseudocubic indices). Moreover, anomalies in the dielectric and ferroelectric response were detected above TTilt which are explained by coupling of short coherence or weakly tilted regions to the ferroelectric polarization. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3580328]“
“Background: Aberrant expression of microRNAs (miRNAs) has been implicated in oncogenesis of various tumors and primary cutaneous T cell lymphomas. Dicer, a ribonuclease III-like enzyme is essential for miRNA processing.

Objective: We initiated a retrospective study to characterize the alterations in the expression profile of Dicer in patients with primary cutaneous T cell lymphomas (CTCL).

In addition to those who are intolerant to clozapine, only 30% to

In addition to those who are intolerant to clozapine, only 30% to 50% experience clinically significant symptom improvement. This review describes the recent evidence for treatment strategies for people not responding to nonclozapine antipsychotic agents and people not responding or only partially responding to clozapine.”

hyponatremia is a critical electrolyte abnormality in allogeneic stem cell transplantation (allo-SCT) recipients and >50% of cases of severe CYT387 mw hyponatremia are caused by the syndrome of inappropriate secretion of antidiuretic hormone (SIADH). Here, we present a patient with rapidly progressive severe hyponatremia as an initial sign and symptom of human herpesvirus-6associated post-transplantation acute limbic encephalitis (HHV-6 PALE) after allo-SCT. A 45-year-old woman with acute lymphoblastic leukemia received unrelated bone marrow transplantation from a one locus-mismatched donor at the DR locus. On day 21, she developed

a generalized seizure and loss of consciousness with severe hyponatremia, elevated serum antidiuretic hormone (ADH), and decreased serum osmolality. A high titer of HHV-6 DNA was detected in cerebrospinal selleck kinase inhibitor fluid. Treatment with foscarnet sodium and hypertonic saline was started with improvement of neurological condition within several days. Although an elevated serum ADH, low serum osmolality, Anlotinib cell line and high urinary osmolality persisted for 2months, she had no other recurrent symptoms of encephalitis. Our experience suggests that hyponatremia accompanied by SIADH

should be recognized as a prodromal or concomitant manifestation of HHV-6 PALE, and close monitoring of serum sodium levels in high-risk patients for HHV-6 PALE is necessary for immediate diagnosis and treatment initiation.”
“Myasthenia gravis (MG) is an autoimmmune disease in which autoantibodies to different antigens of the neuromuscular junction cause the typical weakness and fatigability. Treatment includes anticholinesterase drugs, immunosuppression, immunomodulation, and thymectomy. The autoimmune response is maintained under control by corticosteroids frequently associated with immunosuppressive drugs, with improvement in the majority of patients. In case of acute exacerbations with bulbar symptoms or repeated relapses, modulation of autoantibody activity by plasmapheresis or intravenous immunoglobulins provides rapid improvement. Recently, techniques removing only circulating immunoglobulins have been developed for the chronic management of treatment-resistant patients. The rationale for thymectomy relies on the central role of the thymus. Despite the lack of controlled studies, thymectomy is recommended as an option to improve the clinical outcome or promote complete remission.

Serum cystatin C concentrations were estimated by ELISA during th

Serum cystatin C concentrations were estimated by ELISA during three successive days in neonates treated for infection. The study group consisted of 9 newborns with sepsis, 14 with severe sepsis and 9 with septic shock.

Results/Discussion: At the beginning of the observational

period the mean serum concentration of cystatin C in the study group was 1.35 mg/L (95% CI 1.20-1.49). Surprisingly, the lowest concentration of cystatin was observed in patients with septic AZD6244 concentration shock (1.23 mg/L; 95% CI 0.92-1.54) within the observation period. Higher concentrations were found in neonates with sepsis (1.47 mg/L; 95% CI 1.04-1.90) and severe sepsis (1.50; 1.12-1.87). There was no correlation between serum cystatin C concentration and serum creatinine or gestational age.

A significant cor relation was discovered between chronological age and cystatin C (R=-0.439, p=0.01). There was a tendency for cystatin C to decline during the second observational day in patients with sepsis (to 1.53 mg/L; 95% CI: 1.19-1.86) and severe sepsis (to 1.32 mg/L; 95% CI: 1.07-1.57), while a slight insignificant increase in patient with septic shock (to 1.28 mg/L; 95% CI: 0.88-1.68) was revealed. PF-573228 nmr The interrelation between age and cystatin C concentration disappeared in the following

days of stay in the NICU. Even in patients who died in the course of septic shock the observed changes in cystatin C levels were small and did not exceed those of serum creatinine.

Conclusions: Cystatin C is not a useful marker of kidney function in neonates with sepsis.”
“During 2005-2009, Cilengitide mw a seroepidemiological study was carried out in Croatia to define the population susceptible to common TORCH agents among pregnant and non-pregnant women of childbearing age. The IgG seroprevalence was 29.1% for T. gondii, 94.6% for rubella,

75.3% for cytomegalovirus (CMV), 78.7% for herpes simplex virus type 1 (HSV-1), and 6.8% for HSV-2. Acute toxoplasmosis and CMV infection (positive IgM antibodies with low IgG avidity) were documented in 0.25% and 0.09% women, respectively. IgM prevalence was 1.2% for both HSV-1 and HSV-2. None of the participants showed acute rubella infection. Seropositivity to T. gondii and HSV-2 varied significantly between age groups (p = 0.001 and p = 0.036, respectively). Women residing in rural regions showed a significantly higher seroprevalence rate for T. gondii, CMV, and HSV-1 than urban women (T gondii: 44.0% vs. 25.4%, p < 0.001; CMV: 85.0% vs. 73.1%, p = 0.018; HSV-1: 86.0% vs. 76.4%, p = 0.041).”
“In addition to actual weight loss and the possible resolution of obesity-related co-morbidities following bariatric surgery, another widely recognized important outcome measure is the improvement of quality of life (QOL).

All grafts were still intact but in the early stages of degradati

All grafts were still intact but in the early stages of degradation. No microtubules, indicative of vas deferens recanalization, were identified. One specimen showed evidence

of healing and neovascularization at the interface zone between the vas deferens and the graft. At 12 weeks, grafts were further degraded but still present. Microscopic evaluation showed decreased inflammation. Seven specimens showed neovascularization at the interface zone; two of these showed distinct epithelialized vas deferens microcanals at the graft edges. One specimen showed a microcanal spanning the entire 0.5-cm graft. A time period of 8 weeks is not ample enough for vas deferens regeneration in the setting of a biodegradable PDLA graft; however, early evidence of re-growth was seen LY2603618 supplier at 12 weeks. A longer healing time should permit further biodegradation of the graft, as well as re-growth and possible eventual reconnection

of the vas deferens, allowing passage of sperm. These findings suggest a potential role for biodegradable grafts in the reconstruction of vas deferens with long obstructed segments.”
“We have found that a beryllium (Be) atom on nanostructured materials with H-2 molecules generates a Kubas-like dihydrogen complex [Lee, Huang, Duan, and Ihm, Appl. Phys. Lett. 96, 143120 (2010)]. Here, we investigate the feasibility of Be-decorated fullerenes for hydrogen storage using ab initio calculations. We find that the aggregation of AL3818 nmr Be atoms on pristine fullerenes is energetically preferred, resulting in the dissociation of the dihydrogen. In contrast, for boron (B)-doped fullerenes, Be atoms prefer Flavopiridol to be individually attached to B sites of the fullerenes, and a maximum of one H-2 molecule binds to each Be atom in a form of dihydrogen with a binding energy of similar to 0.3 eV. Our results show that individual dispersed Be-decorated B-doped fullerenes can serve as a room-temperature hydrogen storage medium. (C) 2010 American Institute of Physics. [doi:10.1063/1.3387750]“
“This study assessed the therapeutic effect of and adverse reactions to pirarubicin (THP) chemotherapy in osteosarcoma patients with lung metastasis, and analyzed

the relationship between THP therapeutic effect and expression of P-glycoprotein and topoisomerase-II. Osteosarcoma patients with lung metastases at relapse were given THP and then cisplatin (DDP) or ifosfamide (IFO). Overall survival in patients receiving THP was 31.00 +/- 7.98 months, progression-free survival was 13.00 +/- 2.46 months. Objective response and partial response rates were 46.88% and 40.63%, respectively. There were no differences in overall survival and progression-free survival between the THP+DDP and THP+IFO regimens. Adverse reactions to THP chemotherapy were mainly gastrointestinal and myelosuppression. The therapeutic effect of THP was correlated with the abrogated expression of P-glycoprotein and/or topoisomerase-II positive expression.

Preoperative imaging, clinical characteristics, management decisi

Preoperative imaging, clinical characteristics, management decisions, operative procedures, and outcomes were reviewed.

Results. EPZ015666 Epigenetics inhibitor Eighteen patients with TSC underwent 22 primary minor

resections for SEGAs. The indication for surgery was meaningful radiographic tumor progression in 16 of 21 cases. The average age at the time of operation was 10.3 years. Average follow-up duration was 52 months (range 12-124 months). The operative approach was intrahemispheric-transcallosal in 16 cases, transcortical-transventricular in 5, and neuroendoscopic in 1. Nine tumors were on the right, 9 on the left, and 3 were bilateral. Gross-total resection was documented in 16 of 22 cases in our series, with radical subtotal resection achieved in 4 cases, and subtotal resection

(STR) in 2 cases. Two patients had undergone ventriculoperitoneal shunt placement DMXAA research buy preoperatively and 7 patients required shunt placement after surgery for moderate to severe ventriculomegaly. Two patients experienced tumor progression requiring reoperation; both of these patients had initially undergone STR.

Conclusions. The authors present their management strategy for TSC patients with SEGAs. Select patients underwent microsurgical resection of SEGAs with acceptable morbidity. Gross-total resection or radical STR was achieved in 90.9% of our series (20 of 22 primary tumor resections), with no recurrences in this group. Approximately half of our patient series required CSF diversionary procedures. There were no instances

of permanent neurological morbidity associated with surgery.”

This study aims to introduce a training program for microvascular anastomosis that includes an in vivo model which is elegant and effective.

Material & Methods:

The infrarenal abdominal aorta or inferior vena cava of anesthetized live Sprague-Dawley rats were dissected, clamped and divided. Two trainees each created 12 microsurgical anastomoses in six animals. The training effect find more was measured by comparing the time taken to perform the initial anastomoses with the final attempts for each trainee. Afterwards, each anastomosis was probed to check whether the procedure was successful.


The training model was practical and easy to set up. The average diameter of the aorta measured 1.5 mm and the vein measured 2.1 mm, which reflects the situation in human gynecological reconstructive surgery. The achieved training effect over the course of the training was highly significant. Arterial anastomosis time improved by an average of 8.5 min (P < 0.01) and venous anastomosis time improved by an average of 13.2 min (P < 0.01). The success rate for arterial anastomoses was 67%. In contrast, venous anastomosis was a more difficult exercise, with a success rate of 36% (P < 0.05).


The study establishes a practicable in vivo model of microvascular anastomotic training for gynecologists.

The results suggest that LED therapy is more

efficient th

The results suggest that LED therapy is more

efficient than CWI in preventing muscle damage and local inflammation after exercise.”
“The objective of the study was to investigate the alcoholic (ALEBF) extract of B. flabellifer for their hypoglycaemic effects in normal and diabetic rats. Diabetes was induced in rats by single dose administration of alloxan (120 mg/kg, i.p.) or by injecting dexamethasone (10 mg/kg, i.p.) for 10 days. In normal rats, ALEBF (100, 200 and 400 mg/kg) had significantly decreased the blood glucose level in a dose dependent manner after repeated administration for 7 days. In alloxan induced diabetic rats, extract (ALEBF) had decreased blood sugar level and improved glucose tolerance in alloxan induced diabetic rats at the end of 1st, 2nd, 3rd and 4th week after test extract treatment. NCT-501 research buy However, the insulin levels of extract treated group did not significantly change after 28 days treatment with the extract. It did not alter the insulin levels. Wnt tumor In alloxan model, repeated dose administration of ALEBF had showed significant increase

in body weight, prevention of elimination of sugar in urine and reduced the mortality rate induced by alloxan. In dexamethasone induced insulin resistance diabetic rats, repeated administration of ALEBF inhibited the increase in blood glucose level, improved glucose tolerance and reduced the insulin levels as compared dexamethasone induced diabetic rats.”
“Nephrogenic systemic fibrosis (NSF) is a fibrotic disease that presents with a history of renal dysfunction. The differential diagnosis generally includes scleromyxedema, systemic sclerosis, and morphea. Especially, scleromyxedema can be extremely difficult to distinguish microscopically. Although the fibrocytes in NSF are often positive for CD34 and procollagen-I, this is not specific for NSF. We identified positive Ro-3306 cell line iron staining in the skin of a patient with

NSF and investigated whether this was a specific feature among 9 patients with NSF reported in Japan. We found that 6 of 9 patients showed positive iron staining in the dermal fibrocytes. The amount of iron deposition seemed to have no correlation with the degree of fibrosis or duration of the skin lesions but correlated with apparent history of the use of gadolinium-based contrast agents. As controls, skin biopsies from patients with scleromyxedema, morphea, and systemic sclerosis were evaluated by iron staining. None of these control patients showed iron deposition, indicating that positive iron staining may be specific to NSF and can be a useful tool for NSF diagnosis.”
“The objective assessment of sperm function increases the chances of predicting the fertilizing capacity of a fresh semen sample or diagnosing infertility problems. In this study, the available flow cytometry technique was used to determine the membrane functional capacity of canine spermatozoa.

Results Cold lateral and vertical

Results. Cold lateral and vertical selleck kinase inhibitor condensation had significantly less fluid movement than the Thermafil and Ultrafil groups. Thermafil group had the highest fluid movement values when compared with the other groups (P < .01).


Hybrid Root SEAL (MetaSEAL) had less fluid movement with cold lateral and vertical condensation techniques when compared with Thermafil and Ultrafil techniques. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:e113-e116)”
“In this paper, we developed a rapid and accurate method for the detection of Vibrio parahaemolyticus strains, using multiplex PCR and DNA-DNA hybridization. Multiplex PCR was used to simultaneously amplify three diagnostic genes (tlh, tdh and fla) that serve as molecular markers of V. parahaemolyticus. Biotinylated PCR products were hybridized to primers immobilized on a Eltanexor nmr microarray, and detected by chemiluminesce with

avidin-conjugated alkaline phosphatase. With this method, forty-five samples were tested. Eight known virulent strains (tlh(+)/tdh(+)/fla(+)) and four known avirulent strains (tlh(+)/tdh(-)/fla(+)) of the V. parahaemolyticus were successfully detected, and no non-specific hybridization and cross-hybridization reaction were found from fifteen closely-related strains (tlh(-)/tdh(-)/fla(+)) of the Vibrio spp. In addition, all the other eighteen strains of non-Vibrio bacteria (tlh(-)/tdh(-)/fla(-)) gave negative results. The DNA microarray successfully distinguished V. parahaemolyticus from other Vibrio spp. The results

demonstrated that this was an efficient and robust method for identifying virulent strains of V. parahaemolyticus.”
“A novel conductive imprinted polyaniline (PAN) film is prepared by adding template during the PAN film preparation. Monochloroacetic acid (MCA) and trichloroacetic acid (TCA) were used as selleck templates. The conductivity of imprinted PAN films was measured by the four-point probe method. The conductivity changes of imprinted PAN films were compared to reference PAN reflecting the MCA and TCA specific sites on the surface of PAN films. The conductivities were linearly dependent on the template concentrations, and linear calibration curves were obtained in the range 1-30 and 1-40 ppm of the MCA and TCA, respectively. Excellent method reproducibility (standard deviation 0.04 S/cm(-1)) was observed for the determination of 15 ppm MCA. The effect of various factors on preparation, properties, and recognition effects of the imprinted PAN films was investigated. The best electrical and mechanical properties were obtained with 7 x 10(-4) mmol MCA as a template and doping agent. The measurements are carried out under room temperature, and the maximum conductivities are reached after about 10 and 20 min for reference and imprinted PAN film, respectively.