Results. Cold lateral and vertical selleck kinase inhibitor condensation had significantly less fluid movement than the Thermafil and Ultrafil groups. Thermafil group had the highest fluid movement values when compared with the other groups (P < .01).

Conclusion.

Hybrid Root SEAL (MetaSEAL) had less fluid movement with cold lateral and vertical condensation techniques when compared with Thermafil and Ultrafil techniques. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:e113-e116)”
“In this paper, we developed a rapid and accurate method for the detection of Vibrio parahaemolyticus strains, using multiplex PCR and DNA-DNA hybridization. Multiplex PCR was used to simultaneously amplify three diagnostic genes (tlh, tdh and fla) that serve as molecular markers of V. parahaemolyticus. Biotinylated PCR products were hybridized to primers immobilized on a Eltanexor nmr microarray, and detected by chemiluminesce with

avidin-conjugated alkaline phosphatase. With this method, forty-five samples were tested. Eight known virulent strains (tlh(+)/tdh(+)/fla(+)) and four known avirulent strains (tlh(+)/tdh(-)/fla(+)) of the V. parahaemolyticus were successfully detected, and no non-specific hybridization and cross-hybridization reaction were found from fifteen closely-related strains (tlh(-)/tdh(-)/fla(+)) of the Vibrio spp. In addition, all the other eighteen strains of non-Vibrio bacteria (tlh(-)/tdh(-)/fla(-)) gave negative results. The DNA microarray successfully distinguished V. parahaemolyticus from other Vibrio spp. The results

demonstrated that this was an efficient and robust method for identifying virulent strains of V. parahaemolyticus.”
“A novel conductive imprinted polyaniline (PAN) film is prepared by adding template during the PAN film preparation. Monochloroacetic acid (MCA) and trichloroacetic acid (TCA) were used as selleck templates. The conductivity of imprinted PAN films was measured by the four-point probe method. The conductivity changes of imprinted PAN films were compared to reference PAN reflecting the MCA and TCA specific sites on the surface of PAN films. The conductivities were linearly dependent on the template concentrations, and linear calibration curves were obtained in the range 1-30 and 1-40 ppm of the MCA and TCA, respectively. Excellent method reproducibility (standard deviation 0.04 S/cm(-1)) was observed for the determination of 15 ppm MCA. The effect of various factors on preparation, properties, and recognition effects of the imprinted PAN films was investigated. The best electrical and mechanical properties were obtained with 7 x 10(-4) mmol MCA as a template and doping agent. The measurements are carried out under room temperature, and the maximum conductivities are reached after about 10 and 20 min for reference and imprinted PAN film, respectively.