Stroma anatomy: Ostioles (79–)81–103(–124) μm long, plane or projecting to 20(–23) μm, (28–)32–45(–56) μm wide at the apex inside (n = 30), apical cells cylindrical or terminally slightly widened to 4 μm. Perithecia (165–)185–235(–270) × (115–)130–185(–210) μm (n = 30), flask-shaped or globose; peridium (11–)13–20(–26) μm (n = 30) thick at selleck chemicals llc the base, (4–)8–16(–17) μm (n = 30) laterally; yellow, orange in KOH. Cortical layer (11–)14–20(–23) μm (n = 30) thick, a t. angularis of cells (5–)7–15(–19) × (3–)5–10(–14) μm (n = 60) with walls 0.5–1.3 μm

thick in face view and in vertical section; yellow, orange in KOH. Subcortical tissue a hyaline t. angularis of thin-walled cells (4–)6–11(–16) × (3–)4–7(–8) μm (n = 33), mixed with scant hyphae. Subperithecial tissue a t. angularis–epidermoidea of thin-walled cells (5–)11–22(–27) × (4–)9–16(–19) μm (n = 30), tending to be smaller towards the stroma base. Asci (110–)116–127(–135) × (5.8–)6.3–7.5(–8.0) μm, stipe (10–)15–28(–40) μm long (n = 43); croziers present. Ascospores hyaline, verrucose or spinulose with Selleck JNK-IN-8 spines to nearly 1 μm long, cells distinctly dimorphic; distal

cell (4.3–)5.0–6.8(–9.0) × (3.3–)3.8–4.5(–5.3) μm, l/w (1.1–)1.2–1.7(–2.3) see more (n = 90), subglobose, ellipsoidal or wedge-shaped; proximal cell (4.0–)5.3–7.8(–10.0) × (2.8–)3.5–4.0(–4.5) μm, l/w (1.2–)1.5–2.1(–3.2) (n = 90), oblong or wedge-shaped. Anamorph associated with stromata effuse, hairy, light to dull greyish green, mostly 25DE4–5. Cultures and anamorph: optimal growth at 25°C on all media; hyphae autolysing

and dying after a few days at 30°C; no growth at 35°C. On CMD after 72 h 22–23 mm at 15°C, 42–43 mm at 25°C, 1–3 mm at 30°C; mycelium covering the plate after 5 days at 25°C. Colony hyaline, thin, loose, indistinctly zonate, mycelium radially arranged, scant on Liothyronine Sodium the agar surface, with conspicuous difference in width between wide primary and narrow secondary hyphae; with long and high, loosely arranged aerial hyphae in a broad marginal zone; surface slightly downy, numerous helical hyphae in the centre within the agar. Autolytic excretions absent or inconspicuous, abundant and yellowish at 30°C, coilings inconspicuous. No diffusing pigment, no distinct odour noted. Chlamydospores noted after 7–9 days, rare. Conidiation starting after 4 days, first effuse, sessile and on aerial hyphae, scant, ill-defined, verticillium- to mostly gliocladium-like, conidia produced in wet to dry heads up to 25 μm diam. After collapse of the effuse conidiation, pustules 0.5–2.4 mm diam appearing mostly in distal areas, green after ca 2 weeks; often with white margins and sterile or fertile, straight to subhelical elongations. Formation of pustules not reproducible, absent after a few transfers. At 15°C no conidiation and no chlamydospores seen within 2 weeks. On PDA after 72 h 18–19 mm at 15°C, 34–35 mm at 25°C, 1–2 mm at 30°C; mycelium covering the plate after 6 days at 25°C.

[http://​www.​hotthyroidology.​com] Hot Thyroidology 2005. 32. Tognella C, Marti U, Peter HJ, Wagner HE, Glaser C, Kampf J, Simon F, Hauselmann HJ, Paulsson M, Ruchti C, et al.: Follicle-forming cat thyroid cell lines synthesizing extracellular matrix and basal membrane components: a new tool for the study of thyroidal morphogenesis. J Endocrinol 1999, 163:505–514.PubMedCrossRef 33. Beech SG, Walker SW, Dorrance AM, Arthur JR, Nicol F, Lee D, Beckett GJ: The role of thyroidal type-I iodothyronine

deiodinase in tri-iodothyronine production by human and sheep thyrocytes in primary culture. J Endocrinol 1993, 136:361–370.PubMedCrossRef 34. Donda A, Javaux F, Van Renterghem P, Gervy-Decoster C, Vassart G, Christophe D: Human, bovine, canine and rat thyroglobulin promoter sequences display species-specific differences in an in vitro study. Mol Cell Endocrinol 1993, 90:R23–26.PubMedCrossRef 35. Goffart JC, Dumont JE, Mircescu H: What makes a Selleck FK228 thyroid cell a thyroid cell ? [http://​www.​hotthyroidology.​com/​editorial_​78.​html] Hot Thyroidology 2000. 36. Svenson M, Kayser L, Hansen MB, Rasmussen AK, Bendtzen

K: Interleukin-1 E7080 research buy receptors on human thyroid cells and on the rat thyroid cell line FRTL-5. Cytokine 1991, 3:125–130.PubMedCrossRef 37. Gossrau R, Graf R: Protease cytochemistry in the murine rodent, guinea-pig and marmoset placenta. Histochemistry 1986, 84:530–537.PubMedCrossRef CP673451 38. Kotani T, Aratake Y, Ogata Y, Umeki K, Araki Y, Hirai K, Kuma K, Ohtaki S: Expression of dipeptidyl aminopeptidase IV activity in thyroid carcinoma. Cancer Lett 1991, 57:203–208.PubMedCrossRef 39. Hadler-Olsen E, Fadnes B, Sylte I, Uhlin-Hansen L, Winberg JO: Regulation of matrix metalloproteinase activity in health and disease. FEBS J 2011, 278:28–45.PubMedCrossRef 40. Turk B, Turk D, Salvesen GS: Regulating cysteine protease

activity: essential role of protease inhibitors as guardians and regulators. Curr Pharm Des 2002, 8:1623–1637.PubMedCrossRef 41. van der Hoorn RA, Leeuwenburgh MA, Bogyo M, Joosten MH, Peck SC: Activity profiling of papain-like cysteine proteases in plants. Plant Physiol 2004, 135:1170–1178.PubMedCrossRef 42. Boonacker E, Van Noorden CJ: The multifunctional or moonlighting protein CD26/DPPIV. European journal of cell biology 2003, 82:53–73.PubMedCrossRef Ketotifen 43. St Leger RJ, Cooper RM, Charnley AK: Analysis of aminopeptidase and dipeptidylpeptidase IV from the entomopathogenic fungus Metarhizium anisopliae. J Gen Microbiol 1993, 139:237–243.PubMedCrossRef 44. Gossrau R, Lojda Z: Study on dipeptidylpeptidase II. Histochemistry 1980, 70:53–76.PubMedCrossRef 45. Bernier-Valentin F, Trouttet-Masson S, Rabilloud R, Selmi-Ruby S, Rousset B: Three-dimensional organization of thyroid cells into follicle structures is a pivotal factor in the control of sodium/iodide symporter expression. Endocrinology 2006, 147:2035–2042.PubMedCrossRef 46.

Custom-synthesized oligonucleotides for the PCR were purchased from GeneDesign (Osaka, Japan). DNA sequencing and

informatic analysis To sequence the DNA fragments amplified by PCR, the fragments were purified with the PCR Gel Extraction Kit (QIAGEN, Valencia, CA) according to the manufacturer’s protocol. DNA sequencing was performed with the ABI PRISM 3130 (Applied Biosystems, Foster City, CA) and the BigDye v3.1 cycle sequencing kit (Applied Biosystems). The Genetyx sequence analysis program (Software Development, Tokyo, Japan) was used for computer analysis of DNA sequences. Homology searches against deposited sequences were performed with the aid of data from the National Center for Biotechnology Information see more (NCBI) using the BLAST network service http://​www.​ncbi.​nlm.​nih.​gov and the BLAST service at the Genome Information Research Center http://​genome.​naist.​jp/​bacteria/​vpara/​. Sequence information was obtained from the NCBI. The computer program CLUSTAL W was used for the nucleotide sequence alignment and phylogenetic analysis. Construction of vscN2 deletion selleck products mutant strains of V. mimicus A four-primer PCR technique was used to engineer an in-frame deletion mutation as described previously [14]. Briefly, the upstream and downstream sequences of vscN2 of T3SS2α or T3SS2β were amplified using the pairs listed

in Additional file 1. The two fragments, amplified with primers 1 and 3, and 2 and 4, respectively, were used as templates for a second PCR using primers 1 and 4, which generated a PCR product containing the desired deletion. The amplified fragments were then sequenced and subcloned into an R6K-ori suicide vector pYAK1 and transformed into E. Paclitaxel in vitro coli SM10λpir. CX-4945 cytotoxicity assays For cytotoxicity assays, eukaryotic cells were seeded at

3 × 104 cells well-1 in 96-well plates and cultured for 48 h to confluency. The cells were co-cultured with PBS-washed bacteria at a multiplicity of infection (moi) of 10 for 2- 6 h. The release of lactate dehydrogenase (LDH) into the medium was quantified by using a CytoTox96 non-radioactive cytotoxicity kit (Promega) according to the manufacturer’s instructions. The LDH release (per cent cytotoxicity) was calculated with the following equation: [optical density at 492 nm (OD492) of experimental release - OD492 of spontaneous release]/(OD492 of maximum release – OD492 of spontaneous release) × 100. Spontaneous release is defined as the amount of LDH released from the cytoplasm of uninfected cells, and maximum release as the total amount of LDH released after the complete lysis of uninfected cells. Statistical analysis Statistical significance was determined with the t test. A P value of < 0.05 was considered statistically significant.

Differential gene expression inside MK0683 the ESAT-6 cluster could be related to the presence of the internal promoter pr2, whose activity diminishes under acid stress. As pr2 seems to be a weak promoter, its effect in M. MX69 order tuberculosis could be less evident, while in M. smegmatis it could effectively reduce pr2-regulated genes expression. Unfortunately, it was not possible to identify pr2 promoter sequence in M. tuberculosis, as 5′ RACE experiments were unsuccessful; the probable reason is low expression levels. In M. smegmatis, no SigA consensus sequence could be

found upstream of the 5′ end of the transcript. We can hypothesize the involvement of an alternative sigma factor; indeed, this region showed sequence (boxed in Figure 2B) that resembled the sequence

putatively recognized by M. tuberculosis SigH [19, 34]. However, in this organism, SigH is induced by heat shock and oxidative stress [34] and we are accordingly unclear as to the 4SC-202 cell line meaning of this observation. On the other hand, a bioinformatics search has predicted the existence of 26 sigma factors in M. smegmatis, with a significant enrichment in the SigH subfamily [35]. These paralogous members might have acquired specific functions, and might be induced in varying as yet unidentified conditions. Conclusion Our data suggest that ESAT-6 cluster 3 regulation in mycobacteria varies. Particularly, in M. tuberculosis the gene cluster is induced by iron and zinc starvation and is repressed by IdeR and Zur regulators. In M. smegmatis, only IdeR-dependent regulation is retained,

while zinc has no effect on gene expression. Differences in expression could be due to diversity in the life styles of these organisms. Iron is a limiting growth factor in the environment and during human infection, but as a pulmonary pathogen M. tuberculosis also contend with a zinc-deficient environment. Although the role of cluster 3 is not defined, induction in iron- and zinc-deficient condition, as pertain in the lung, strongly suggests a high level expression of this cluster during the infective process. Both in M. tuberculosis Inositol monophosphatase 1 and in M. smegmatis we identified an internal promoter just upstream of the esx genes (respectively rv0287 and msmeg0620). These promoters seem to be repressed under acid stress, and thus to contribute to differential expression of this gene cluster in varying environmental conditions. Methods Strains, media and growth conditions Escherichia coli XL1-Blue was grown in Luria Bertani (LB) medium [36] at 37°C. When required, antibiotics were added at the following concentrations: ampicillin, 100 μg/ml; streptomycin, 50 μg/ml, tetracycline, 12.5 μg/ml. M.

In this study, the GSK2399872A Cthe1053 gene displayed low expression and lactate was not detected during cellulose fermentation. Although another gene annotated as ldh (Cthe0345) was expressed at high levels, this may be related to the participation of the encoded enzyme as a malate dehydrogenase in the alternate route

for conversion of PEP to pyruvate, as discussed earlier (Figure 4). Pyruvate Pexidartinib formate lyase (pfl) catalyzes the conversion of pyruvate to formate, along with the formation of acetyl-CoA. Sparling et al, reported formate synthesis in C. thermocellum via this pathway with detection of transcripts for pfl (Cthe0505) and the pfl activating enzyme (Cthe0506) by RT-PCR [13]. In this study, two out of four putative pfl activating enzymes (Cthe0506, Cthe0647) were expressed at relatively high levels during cellulose fermentation (Additional file 5; data not available for pfl, Cthe0505). However, formate was not detectable

in FK228 manufacturer the culture supernatant consistent with other previous reports [25, 28]. Acetyl-CoA is further catabolized to acetate with generation of ATP or to ethanol with reoxidation of NADH. C. thermocellum encodes an NADH-dependent aldehyde dehydrogenase (aldH, Cthe2238), which catalyzes the conversion of acetyl-CoA to acetaldehyde, and several iron-containing alcohol dehydrogenases (Cthe0101, Cthe0394 [adhY] and Cthe2579 [adhZ]) for alcohol synthesis from acetaldehyde; also encoded is a bi-functional acetaldehyde/alcohol dehydrogenase (Cthe0423, adhE) which catalyzes the direct conversion of acetyl-CoA to ethanol (Figure 4). AdhE has been proposed to be a key enzyme for ethanol synthesis in C. thermocellum Idoxuridine and transcription

of adhE, adhY and adhZ has been confirmed by RT-PCR analysis in cellobiose and cellulose cultures of C. thermocellum [11, 19]. In this study, the aldH gene showed increased expression during stationary phase while the three adh genes, Cthe0394, Che0423 and Cthe0101, were actively expressed during cellulose batch fermentation with the latter showing decreased expression in stationary phase (Figure 4, Additional file 5). Acetyl-CoA is indirectly converted to acetate via acetyl-phosphate through the action of two enzymes, encoded by the contiguous genes, phosphotransacetylase (pta, Cthe1029) and acetate kinase (ack, Cthe1028), with the generation of one ATP per acetate molecule. The reverse reaction for direct conversion of acetate to acetyl-CoA utilizes ATP and is catalyzed by acetyl-CoA synthetase (acs, Cthe0551). Previous studies have confirmed the expression of acetate kinase through RT-PCR [11] and enzyme activity measurements [25]. In this study, both pta and ack genes were expressed at low levels which further decreased in stationary phase; whereas, the acs gene was expressed at relatively higher levels over the entire course of the fermentation (Figure 4, Additional file 5).

Serum LDL decreased slightly in response to creatine loading in the CrM group but returned to baseline after ingesting maintenance doses of CrM suggesting these changes were transient. Additionally, no significant differences were observed among groups in markers of catabolism (BUN, BUN:CRN, AST, ALT, Total Protein, TBIL), markers of bone status (bone mineral content, ALB, GLOB, ALB:GLOB, calcium, ALK) or whole blood markers (WBC, RBC, Hematocrit, SRT2104 in vivo Hemoglobin, MCV, MCH, MCHC, RBCDW, platelet counts). Moreover, values remained within normal levels for active individuals. These findings are consistent with other studies that have examined the safety of creatine supplementation in active individuals

[1, 3, 21, 26, 27, 38]. Consequently, present findings do not support claims AZD8931 clinical trial that KA is a safer form of creatine to ingest than creatine monohydrate.

Conclusion In summary, supplementation of the diet with recommended doses of a purported buffered form of creatine (1.5 g/d) for 28-days or equivalent loading (20 g/d for 7-days) and maintenance doses (5 g/d for 21-days) of CrM did not promote greater increases in muscle creatine content or training adaptations in comparison to creatine monohydrate (20 g/d for 7-days, 5 g/d for 21-days). Additionally, there was no evidence to support claims that the buffered form of creatine was associated with fewer side effects or was a safer form of creatine to consume than creatine monohydrate. While it could be argued that supplementing the diet with any form of creatine may provide some health and/or PI-1840 ergogenic benefits over time as long as it delivers sufficient amounts of creatine to increase muscle creatine content; present findings do not support claims

that KA is a more efficacious and/or safer form of creatine than creatine monohydrate. With this said, some selleckchem limitations of this study should be noted. For example, this study did not have a control group and depended on participants to self-report side effects. Therefore, while the safety profile of short and long-term creatine monohydrate supplementation has been well established, safety and efficacy could only be compared to ingesting different levels and forms of creatine and not controls. There is also variability in conducting muscle and blood assays as well as variability in conducting performance tests. In some instances, large mean differences among groups were either not statistically significant or only approached significance. It is possible that some of these differences would have been significant if a control group was included in the study design and/or more subjects were studied to increase statistical power. Nevertheless, results from the present study do not support claims that KA is a more efficacious and/or safer form of creatine to consume than creatine monohydrate. Funding Supported by AlzChem AG, Germany.

Biol Conserv 148:180–190. doi:10.​1016/​j.​biocon.​2012.​01.​014 CrossRef Lewin I (2006) The gastropod communities in the lowland rivers of agricultural areas—their biodiversity and bioindicative value in the Ciechanowska Upland, Central Poland. Malacologia 49:7–23CrossRef Lewin I, Smoliński A (2006) Rare, threatened and alien species

in the gastropod communities in the clay pit ponds in relation to the environmental factors (The Ciechanowska Upland, Central Poland). Biodivers Conserv 15:3617–3635. doi:10.​1007/​s10531-005-8347-4 CrossRef Lipsey L, Malcolm S (1981) Summer zooplankton communities of selected borrow-pit ponds in Northern Illinois. Hydrobiologia 77:81–85CrossRef Majewski T (1998) New and rare Hydraenidae i Hydrochidae (Coleoptera) w Polsce. Acta

entomol silesiana 5–6:21–23 Menetrey CB-839 N, Sager L, Oertli B, Lachavanne JB (2005) Looking for metrics to assess the trophic state of ponds. Macroinvertebrates and amphibians. Aquat Conserv learn more Mar Freshw Ecosyst 15:653–664CrossRef Ohnesorge D (1988) Die Libellenfauna (Odonata) der Kiesgrube Barkholz (Kreis Stormarn, Schleswig—Holstein). Seevögel 9:17–25 Ott J (1995) Die Beeinträchtigung von Sand- und Kiesgruben durch intensive Angelnutzung—Auswirkungen auf die Libellenfauna und planerische Lösungsansätze. Limnol aktuell 7:155–170 Pakulnicka J (2004) The aquatic beetles in post-exploitation water bodies in Poland. Latissimus 18:22–26 Pakulnicka J (2008) The formation of water beetle fauna in anthropogenic water bodies. Oceanol STA-9090 chemical structure Hydrobiol Stud 37:31–42. doi:10.​2478/​v10009-007-0037-y CrossRef Pakulnicka J, Biesiadka E (2011) Adenosine Water beetles fauna of Olsztyn (Poland). In: Indykiewicz P et al. (eds) Urban fauna. Studies of animal biology, ecology and conservation in the European Cites. University of Technology and Life Sciences, Bydgoszcz, pp 305–317 Pakulnicka J, Nowakowski JJ (2012) The

effect of hydrological connectivity on water beetles fauna in water bodies within the floodplain of a lowland river (Neman river, Belarus). Oceanol Hydrobiol Stud 41:7–17. doi:10.​2478/​s13545-012-0012-4 CrossRef Pakulnicka J, Zawal A (2007) Chrząszcze wodne (Coleoptera) rezerwatu jezioro Szare i jego otuliny. Parki nar Rez Przyr 26:121–133 Pakulnicka J, Eyre M, Czachorowski S (1998) Materials to the knowledge of water and semiaquatic beetles (Coleoptera) if the vicinity of Olsztyn. Wiad Entomol 17:69–74 Pawłowski J, Kubisz D, Mazur M (2002) Coleoptera Chrząszcze. In: Głowaciński Z (ed) Red list of threatened animals in Poland. Polish Academy of Sciences, Institute of Nature Conservation, Cracow, pp 88–110 Przewoźny M (2004) New records of the Hydrophiloidea (Coleoptera: hydrophiloidea) w Polsce.

Fungal Ecol 3(3):240–254CrossRef Goldman N, Yang Z (1994) A codon-based model of nucleotide substitution for protein-coding DNA sequences. Biol Evol 11:725–736 Gond SK, Verma VC, Kumar A, Kumar V, Kharwar RN (2007) Study of endophytic fungal community from different parts of Aegle marmelos Correae (Rutaceae) from Varanasi (India). World J Microbiol Biotechnol 23:1371–1375CrossRef Hallé F, Martin R (1968) Etude de la croissance rythmique chez l’hévéa (Hevea brasiliensis) (Müll. Arg., Euphorbiacées, crotonoïdées). Adansonia 8:475–503 Hyde KD, Ho WH, McKenzie EHC, Dalisay T (2001) Saprobic fungi on bamboo culms. Fungal Divers 7:35–48 Jayasinghe

CK, Silva WPK (1996) Current status of Corynespora leaf fall in Sri Lanka. In: Proceeding on the Workshop on Corynespora Leaf Fall Disease, MX69 Medan, Indonesia, pp 3–5 Jayasinghe

HDAC inhibitor CK, Silva WPK, Wettasinghe DS (1998) Corynespora cassiicola: a fungal pathogen with diverse symptoms on Hevea rubber. Bull Rubber Res Inst Sri Lanka 39:1–5 Junqueira NTV, Gasparotto L, Moraes VHF, Silva HM, Lim TM (1985) New diseases caused by virus, fungi and also bacterium on rubber from Brazil and their impact on international quarantine. In: Proceeding of the regional conference on plant quarantine support for selleck chemicals llc agricultural development, Kuala Lumpur, Malaysia, 10–12 December, pp 253–260 Kingsland GC (1985) Pathogenicity and epidemiology of Corynespora cassiicola in the Republic of the Seychelles. Acta Hortic (ISHS) 153:229–230 Kodsueb R, MacKenzie EHC, Lumyong S, Hyde KD (2008) Diversity of saprobic fungi on Magnoliaceae. Fungal Divers 30:37–53 Koenning SR, Creswell TC, Dunphy EJ, Sikora EJ, Mueller JD (2006) Increased occurrence of target spot of soybean caused by Corynespora cassiicola in the Southeastern United States. Plant Dis 90(7):974. doi:10.​1094/​PD-90-0974C CrossRef Krogh A, Larsson B, von Heijne Baricitinib G, Sonnhammer ELL (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305:567–580PubMedCrossRef Kumar D, Hyde K (2004) Biodiversity

and tissue-recurrence of endophytic fungi in Tripterygium wilfordii. Fungal Divers 17:69–90 Lana T, Azevedo J, Pomella A, Monteiro R, Silva C, Araujo W (2011) Endophytic and pathogenic isolates of the cacao fungal pathogen Moniliophthora perniciosa (Tricholomataceae) are distinguishable based on genetic and physiological analysis. Genet Mol Res 10(1):326–334PubMedCrossRef Lee S, Melnik V, Taylor J, Crous P (2004) Diversity of saprobic hyphomycetes on Proteaceae and Restionaceae from South Africa. Fungal Divers 17:91–114 Liyanage NIS, Liyanage AS (1986) A study on the production of toxin in Corynespora cassiicola. J Rubber Res Inst Sri Lanka 65:51–53 Liyanage AS, Jayasinghe CK, Liyanage NIS, Jayaratne AHR (1986) Corynespora Leaf spot disease of rubber (Hevea brasiliensis)—a new report.

2000). Furthermore, low Taxol concentrations, comparable to the levels we detected in endophyte extracts, did not affect the physiological properties of the membrane (Balasubramanian and Straubinger 1994; Sharma and Straubinger 1994; Bernsdorf et al. 1999; Crosasso et al. 2000; Zhao and Feng 2004). Although these experiments involved selleck artificial membranes, there is also evidence

that fungi can take up non-polar compounds by passive transport and store them in vesicles. For example, Fusarium solani can absorb polyaromatic compounds from the cell culture medium and store them within intracellular compartments with no impact on growth (Verdin et al. 2005). In the endophytes we studied, the accumulation of non-polar taxoid molecules in lipophilic cell structures combined with the high sensitivity of our analytical methods, immunological detection and LC/MS/MS-based multi-reaction monitoring (MRM) ensured that these check details carry-overs could be detected. After the first and second passages of the fungal cultures, no taxanes could be detected by LC/MS/MS. The fungi were no longer associated with the Taxol source and DMXAA hence the trace amounts of taxanes detected initially were diluted below the detection limit. Our

results and conclusions therefore offer a satisfactory explanation for the contradictory results in earlier publications, some providing evidence for independent taxane biosynthesis in different endophytic fungi and others lacking this evidence. Acknowledgments U.H. was supported by a pre-doctoral fellowship from the Volkswagen Foundation, Hannover, Germany (AZ.: I/82 754). Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source

are credited. Electronic supplementary material Below is the link to the electronic supplementary material. ESM 1 (DOCX 666 kb) References Agger S, Lopez-Gallego F, Schmidt-Dannert C (2009) Diversity of sesquiterpene synthases in the basidiomycete Coprinus cinereus. Mol Microbiol 72(5):1181–1195PubMedCrossRef Balasubramanian SV, Straubinger RM (1994) Taxol–lipid interactions: taxol-dependent effects on the physical properties of model membranes. Biochemistry Forskolin mw 33:8941–8947PubMedCrossRef Baloglu E, Kingston DGI (1999) Taxane diterpenoids. J Nat Prod 62:1448–1472PubMedCrossRef Bernsdorf C, Reszka R, Winter R (1999) Interaction of the anticancer agent Taxol (paclitaxel) with phospholipid bilayers. J Biomed Mater Res 46:141–149CrossRef Bömke C, Tudzynski B (2009) Diversity, regulation, and evolution of the gibberellin biosynthetic pathway in fungi compared to plants and bacteria. Phytochemistry 70:1876–1893PubMedCrossRef Brown DT (2003) Preclinical and clinical studies of the taxanes. In: Itokawa H, Lee K-H (eds) The genus Taxus.

PubMedCentralPubMedCrossRef 21. Biggins JB, Liu X, Feng Z, Brady SF: Metabolites from the induced expression of cryptic single operons found in the genome of Burkholderia pseudomallei.

J Am Chem Soc 2011, 133(6):1638–1641.PubMedCrossRef 22. Tang H, Braun TF, Blair DF: Motility protein complexes in the bacterial selleck products flagellar motor. J Mol Biol 1996, 261(2):209–221.PubMedCrossRef 23. Cotter PA, Melville SB, Albrecht JA, Gunsalus RP: Aerobic regulation of cytochrome d oxidase (cydAB) operon expression in Escherichia coli: roles of Fnr and ArcA in repression and activation. Mol Microbiol 1997, 25(3):605–615.PubMedCrossRef 24. Lefebre MD, Valvano MA: Construction and evaluation of plasmid vectors optimized for constitutive and regulated gene expression in Burkholderia cepacia complex isolates. Appl Environ Microbiol 2002, 68(12):5956–5964.PubMedCentralPubMedCrossRef 25. Sambrook J, Maniatis T, Fritsch EF: Molecular cloning : a laboratory manual. 2nd edition. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory; 1989. 26. Lodge J, Fear J, Busby S, Gunasekaran P, Kamini NR: Broad host range plasmids carrying the Escherichia coli SBI-0206965 lactose and galactose operons. FEMS Microbiol Lett 1992, 74(2–3):271–276.PubMedCrossRef 27. DeShazer D, Brett PJ, Carlyon R, Woods DE: Mutagenesis of Burkholderia pseudomallei

selleck screening library with Tn5-OT182: isolation of motility mutants and molecular characterization of the flagellin structural gene. J Bacteriol 1997, 179(7):2116–2125.PubMedCentralPubMed 28. Holden MT, Titball RW, Peacock SJ, Cerdeño-Tárraga AM, Atkins T, Crossman LC, Pitt T, Churcher C, Mungall K, Bentley SD, Sebaihia M, Thomson DNA Damage inhibitor NR, Bason

N, Beacham IR, Brooks K, Brown KA, Brown NF, Challis GL, Cherevach I, Chillingworth T, Cronin A, Crossett B, Davis P, DeShazer D, Feltwell T, Fraser A, Hance Z, Hauser H, Holroyd S, Jagels K, et al: Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proc Natl Acad Sci U S A 2004, 101(39):14240–14245.PubMedCentralPubMedCrossRef 29. Shanks J, Burtnick MN, Brett PJ, Waag DM, Spurgers KB, Ribot WJ, Schell MA, Panchal RG, Gherardini FC, Wilkinson KD, Deshazer D: Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun 2009, 77(4):1636–1648.PubMedCentralPubMedCrossRef 30. Teh BE, French CT, Chen Y, Chen IG, Wu TH, Sagullo E, Chiou PY, Teitell MA, Miller JF, Gan YH: Type three secretion system-mediated escape of Burkholderia pseudomallei into the host cytosol is critical for the activation of NFkappaB. BMC Microbiol 2014, 14:115.PubMedCentralPubMedCrossRef 31. Tan KS, Chen Y, Lim YC, Tan GY, Liu Y, Lim YT, Macary P, Gan YH: Suppression of host innate immune response by Burkholderia pseudomallei through the virulence factor TssM. J Immunol 2010, 184(9):5160–5171.PubMedCrossRef 32.