A total of 1510 Gram-negative pathogens were tested and the minimum-inhibitory concentrations

(MICs) were determined by broth microdilution method according to CLSI.

There was no resistance to either imipenem or meropenem observed for Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis in both medical centers. High resistance rates of K. pneumoniae to ceftazidime (18%), cefepime (17%) and gentamicin (39%) are raising concern. Acinetobacter baumannii turned out to be the most resistant Gram-negative bacteria with 81% resistant to ceftazidime, 73% to cefepime, 69% to gentamicin and 71% to ciprofloxacin. Almost 20% of Pseudomonas aeruginosa strains were resistant to imipenem, 13% to meropenem, 4-Hydroxytamoxifen 69% to gentanmicin and 38% to ciprofloxacin.

The prevalence of extended-spectrum beta-lactamases (ESBLs) in E. coli was 10% and in K. pneumoniae 49%. PCR and sequencing of the amplicons revealed the presence of SHV-5 in nine E. coli strains and additional TEM-1 beta-lactamase five strains. Five K. pneumoniae strains were positive for bla(SHV-5) gene. Eight ESBL positive Enterobacter spp. strains were found to produce TEM and CTX-M beta-lactamases. Plasmid-mediated AmpC beta-lactamases were not found among K. pneumoniae, E. coil and Enterobacter spp. Three A. CX-4945 baumannii strains from Zagreb University Center were

JNK signaling pathway 抑制剂s identified by multiplex PCR as OXA-58 like producers. Six A. baumannii strains from Split University Center were found to possess an ISAba1 insertion sequence upstream of bla(OXA-51) gene. According to our results meropenem remains an appropriate antibiotic for the treatment of severe infections caused by Gram-negative bacteria. These data indicate that despite continued use of meropenem, carbapenem resistance is not

increasing among species tested, except for A. Baumannii, in the two study hospitals and suggest that clinicians can still administer carbapenems as a reliable and effective choice in managing serious nosocomial infections.”
“We report synthesis, structural details, and magnetization of SmFe1-xCoxAsO with x ranging from 0.0 to 0.30. It is found that Co substitutes fully at Fe site in SmFeAsO in an isostructural lattice with slightly compressed cell. The parent compound exhibited known as the spin density wave (SDW) character is below at around 140 K. Successive doping of Co at Fe site suppressed the SDW transition for x=0.05 and later induced superconductivity for x=0.10, 0.15, and 0.20, respectively, at 14, 15.5, and 9 K. The lower critical field as seen from magnetization measurements is below 200 Oe. The appearance of bulk superconductivity is established by wide open isothermal magnetization M(H) loops. Superconductivity is not observed for higher content of Co, i.e., x >= 0.30.