Plug becoming rosy, carrot to dark reddish-brown, 7–8EF6–8; colon

Plug becoming rosy, carrot to dark reddish-brown, 7–8EF6–8; colony becoming discoloured from the plug in zones, pale orange, reddish-brown, carrot, to dull orange-brown, 5AB5–6, 6BD5–7. No distinct

odour noted. Conidiation noted after 3 days, effuse on long BAY 73-4506 aerial hyphae, verticillium-like, particularly dense in the centre. At 15°C conidiation reduced, colony turning orange to brown, 5AB4–6 to 7DE7–8, pigment diffusing from pigmented hyphae into the agar. On SNA after 72 h 4–6 mm at 15°C, 11–13 mm at 25°C, 3–5 mm at 30°C; mycelium covering the plate after more than 2 weeks at 25°C. Colonies similar to CMD, but more irregular, marginal hyphae forming pegs. Aerial hyphae abundant, cottony, ascending to the lid of the Petri dish, dichotomously branched, appearing nearly setose with pointed ends. Autolytic excretions scant, coilings frequent. No diffusing pigment, no distinct odour noted. No chlamydospores seen. Conidiation noted after 3–4 days, effuse, white, loose, see more translucent, verticillium-like. Main axes 7–9 μm wide, with walls to 2.5 μm thick and outer layer deliquescent, bearing numerous short, usually unpaired conidiophores, often in right angles. Conidiophores mostly 3–6 μm wide, sometimes widening to 7.5 μm; terminally 2–3 μm wide. Side branches simple or rebranching, 60–160 μm long; tips of side branches with phialides or short, unpaired or paired, 1-celled branches 10–20 μm long, slightly inclined

upwards. Phialides solitary or divergent in whorls of 2–6(–8), arising from cells 2–4(–5.5) μm wide, forming conidia in minute wet heads mostly <20 μm diam. Phialides (8–)11–16(–23) × (2.0–)2.3–3.0(–3.5) μm, l/w (3.3–)4.0–6.6(–10), 1.5–2.5(–3.0) wide at the base (n = 40), narrowly lageniform, subulate or fusoid, widest in or below middle. Conidia (2.6–)3.0–4.0(–5.2) × (2.0–)2.2–2.5(–2.8) μm, l/w 1.2–1.7(–2.2) (n = 50), hyaline, ellipsoidal or oblong, Epothilone B (EPO906, Patupilone) smooth, with several guttules and indistinct scar. Habitat: on wood and bark of

deciduous and coniferous trees, leaves, and moss. Known distribution: Europe (Austria, France, United Kingdom). Lectotype, designated by Rossman et al. (1999): France, Clamart, 4 Jan. 1860, M.L.-R. Tulasne, PC 93188 (PC); fungus on thin twig of Quercus, moss and leaves, soc Mycosphaerella punctiformis on leaves. Epitype here designated in order to connect the morphology with molecular phylogeny: Austria, Osttirol, Lienz, Kals am Großglockner, Teischnitztal, MTB 8941/4, 47°01′46″ N, 12°37′49″ E, elev. 1670 m, on log of Picea abies 14 cm thick at roadside, on ?Tomentellastrum sp. and wood, attacked by a hyphomycete, 5 Sep. 2003, W. Jaklitsch W.J. 2377 (WU 29225, culture CBS 120631 = C.P.K. 1603). Holotype of Trichoderma BIX 1294 purchase delicatulum isolated from WU 29225 and deposited as a dry culture with the epitype of H. delicatula as WU 29225a. Other specimens examined: France, Chaville, 21 Mar. 1860, M.L.-R. Tulasne, PC 93187 (PC); 2 pieces of ?Quercus bark, soc.

Comments are closed.