In the UV-visible spectrum, a strong, broad peak at about 420 nm was observed for AgNPs (Figure 1). The specific and characteristic
features of this peak, assigned to a surface plasmon, has been well documented for various metal nanoparticles with sizes ranging from 2 to 100 nm [27, 28]. Bortezomib in vivo The silver nanoparticles were formed by adding 10 ml leaf extracts with aqueous AgNO3. After 6 h, the color of the mixed solutions of leaf extract and AgNO3 changed from pale green to deep brown indicating the formation of silver nanoparticles. The change in color of the reaction medium as an effect of presence of reducing potential substances present in the leaf extract. The color of the silver nanoparticles are due to excitation of surface plasmon vibration in silver nanoparticles and this color change is due to redox reaction between the leaf extract and AgNO3. AgNPs have free electrons, which give rise to a surface plasmon resonance click here absorption due to the combined vibration of electrons of the metal nanoparticles in resonance with the light wave.  It is observed from Figure 1 that the synthesized AgNPs display a clear and single surface plasmon resonance (SPR) band located at 420 nm which confirms the reduction of silver ion to metallic silver. In contrast, AgNO3 shows maximum
absorbtion at 220 nm, whereas the leaf extract shows two absorbtion peaks at 450 and 650 nm. The sharp absorption peak of AgNPs indicates that the formation of spherical and homogeneous distribution of silver nanoparticles. The similar observation was reported using leaf extract
of Delonix elata mediated synthesis of silver nanoparticles . XRD analysis of AgNPs Further, the synthesized silver nanoparticles were confirmed using XRD analysis. Figure 2 shows that the XRD patterns of natural dried silver nanoparticles synthesized using leaf extract. A number of Bragg reflections with 2θ values of 24.48°, 30.01°, 33.30°, 34.50°, 46.30° sets of lattice planes are observed which may be indexed to the (111), (200), and (220) faces of silver respectively. The XRD pattern thus clearly selleckchem illustrates that the silver nanoparticles formed in this present synthesis are crystalline in nature and having face centered cubic (fcc) crystal Uroporphyrinogen III synthase structure. The XRD pattern confirmed the presence of Ag colloids in the sample. A strong diffraction peak located at 30.01 was ascribed to the (111) facets of Ag. The intensive diffraction peak at a 2θ value of 30.01° from the (111) lattice plane of fcc silver unequivocally indicates that the particles are made of pure silver. Two additional broad bands are observed at 34.50°, 46.30° correspond to the (200) and (220) planes of silver respectively (Figure 2). The Braggs reflections were also observed in the XRD pattern at 2θ = 24.48° and 32.50°. The assigned peaks at 2θ values of 24.48°, 29.0°, and 32.