Most bacteria tend to be attracted to mineral surfaces by chemotaxis [17], and bacterial cells often gather in or focus on the crystal boundaries of ores [18]. Recently, biohydrometallurgical extraction of copper from low-grade chalcopyrite ore, which is quite abundant and widespread in the earth’s crust, especially applied to heap bioleaching, is paid more attention. Many studies and researches have been done for that, while the operation is yet to be applied successfully at industrial and commercial scale, due to the extremely slow leach kinetics and low leaching rate. The problem that causes the delay of the application is commonly

attributed to the passivation on the surface of chalcopyrite [19], [20] and [21]. Sulfur, jarosite, disulfide and polysulfide find more are gotten and identified in the biofilm, while there is no generally accepted theory that can wholly explain

the mechanism of biofilm formation [3], [22], [23] and [24]. Pyrite (FeS2) is the most abundant metal sulfide associated with the earth’s surface BIBF 1120 chemical structure region, which is commonly considered as ‘Fool’s gold’. Pyrite is frequently found in massive hydrothermal deposits, sedimentary beds, veins and replacements, and igneous rock, its reserve is ample and luxuriant, to some extent [25]. Rickard and Luther have estimated that, there is about 5 million tons of pyrite being produced annually in the oceanic environment, simply due to the biogenic reduction of aqueous sulfate [26]. Pyrite is often associated with valuable minerals such as sphalerite, chalcopyrite and galena, and pyrite is commonly used for production of sulfuric acid during the process of leaching [27]. Galvanox™ is frequently referred when the combination of the chalcopyrite and pyrite is used in the leach pulp (slurry) [28] and [29]. The name Galvanox™ is given due to the Fenbendazole galvanic interaction between chalcopyrite and pyrite in ferric sulfate media [30]. Heaps and stirred tanks are two different commercial and engineering applications in terms of biohydrometallurgy of sulfide minerals, based on the mechanisms of

bioleaching and mineral biooxidation, which have been purposefully amended and accurately improved from the traditional metallurgical craft since the mid-1980s. Currently heap leaching accounts approximately for 20% of the worldwide copper production [31] and an estimated about one fifth of the world’s copper produced from run-of-mine and crushed ores through bioleaching heap can be reached. In the process of the heap bioleaching and mineral biooxidation, the ores and minerals, which are pretreated by metallurgical and mining methods and stacked on waterproof layers (polymer materials) are continually irrigated with the mixture of a dilute sulfuric acid solution and acidophilic microorganisms. After a period of time leaching solution that contains the released and enriched metal, are collected at the bottom and transported to the upstream of the traditional metallurgical sections or plants.