For example, considering the model based on the raw spectra, it can be observed that pure coffee samples present negative values for DF1 and DF2 and positive values for DF3, whereas adulterated samples

present negative values for DF1, DF2 and DF3. In the model based on normalized data, the only group that presented positive values for both DF1 and DF2 was pure coffee. Both the developed models (based on raw and normalized spectra) presented 100% recognition and prediction abilities. Such results confirm that DRIFTS provides satisfactory discrimination between roasted coffee and roasted adulterants such as corn and coffee husks. We emphasize PI3K activation that the analysis has been carried out using a representative range of roasting conditions, and that variations in roasting degree and temperature did not affect discrimination. This is particularly interesting, given that such variations have been shown to affect discrimination by chromatographic methods ( Franca et al., 2009; Oliveira et al., 2009). The feasibility of employing DRIFTS as a methodology RAD001 cell line for simultaneous discrimination between roasted coffee and commonly employed adulterants such as coffee husks and corn was evaluated. The obtained spectra were similar, with most of the significant bands concentrated in the following ranges: 3000–2800 and 1800–700 cm−1.

In general, absorbance values were higher for roasted coffee and lower for roasted corn. PCA results based on raw, normalized and first derivatives of spectra indicated separation of the samples into the three specified categories. LDA classification models presented PRKACG recognition and prediction abilities of 100% and were able to provide complete discrimination between roasted coffee, pure adulterants (corn and coffee husks) and adulterated coffee samples. The results obtained in the present

study confirm that DRIFTS presents potential for the development of an analytical methodology for detection of adulteration in roasted and ground coffee. Further studies will be conducted for the detection and discrimination of other commonly used coffee adulterants, such as spent coffee grounds and roasted barley. The authors acknowledge financial support from the following Brazilian Government Agencies: CNPq and FAPEMIG and would like to thank the reviewers for their valuable comments. “
“The role that food industry plays on people’s everyday life is undeniable, as well as the importance of diet on the prevention of diseases and its association to health promotion. Food industry has amplified market by providing practical foods and goods for consumers’ convenience. The association of diet with a healthy attitude leads to the creation of products considered not only healthy but also with good palatability (Ares, Giménez, & Gámbaro, 2009). Products may be developed through the substitution of unhealthy ingredients (e.g.