8 1 707 71 24 5 Hma8N 2.1 2 078 68 29 3 Bma5N 2.0 1 929 69 27 4 Hma2N 2.2 2 066 69 28 3 Biut2N 2.1 2 037 70 27 3 Hma11N 1.7 1 585 71 25 4 NVP-BGJ398 in vitro Bifidobacterium           Bin2N 2.1 1 740 67 27 6 Bin7N 2.1 1 718 69 26 5 Hma3N 2.2 1 836 68 26 6 Bma6N 1.7 1 386 73 23 4 An overview of the results

LY2874455 of extra-cellular peptides and proteins from each LAB during microbial stress is shown in Figure  1 and in Table  2. Each of the 13 species and the extra-cellular proteins they produce are depicted more thoroughly in the Additional file 1: Table S1-S9. Putative identification and function were achieved from searches in NCBI (non-redundant database), InterProScan (default database), and Pfam (default database). We identified a vast range of extra-cellular proteins from 10 of the 13 LAB spp., but the majority of the proteins produced had unknown functions. Most of the identified proteins were enzymes, S-layer proteins, DNA chaperones, bacteriocins, and lysozymes (Table  2). Figure 1 Tricine-SDS-PAGE analysis of extracellular proteins and peptides from some of the LAB strains during stressed and un-stressed conditions. Lane 1- Lactobacillus

Fhon13N stressed with LPS, Lane 2- Lactobacillus Fhon13N stressed with LA, Lane 3- Lactobacillus Fhon13N unstressed, lane 4- L. kunkeei Fhon2N stressed with LPS, lane 5- L. kunkeei Fhon2N stressed with LA, lane 6-L. kunkeei Fhon2N unstressed, lane 7- molecular weight marker, lane 8- Bifidobacterium Bin7N stressed with LPS, lane 9- Bifidobacterium Geneticin Bin7N stressed with LA and lane 10- Bifidobacterium Bin7N unstressed. The second gel is as follows:

Lane 1- Lactobacillus Bma5N stressed with LPS and lane 2- Lactobacillus Bma5N, lane 3- Bifidobacterium Hma3N PDK4 stressed with LPS, lane 4- Bifidobacterium Hma3N unstressed. Marks of X are an indication of where a band was cut and analyzed with MS. Table 2 An overview of all extra-cellular proteins synthesized during stress conditions (LPS, LA), from all 13 LAB spp   Peptides with unknown function Peptides with enzymatic function S-layer proteins Chaperones and stress response proteins Bacteriocins and lysozymes Other Total proteins produced Lactobacillus               Fhon13N 4 4 0 0 1 0 9 Fhon2N 17 3 0 0 1 3 24 Bin4N 5 7 0 1 0 9 22 Hon2N 4 26 0 5 1 10 46 Hma8N 0 0 0 0 0 0 0 Bma5N 0 2 1 0 1 0 4 Hma2N 0 8 2 0 0 2 12 Biut2N 3 0 0 0 0 0 3 Hma11N 0 1 2 1 0 0 4 Bifidobacterium               Bin2N 2 5 0 0 0 0 7 Bin7N 0 0 0 0 0 0 0 Hma3N 5 4 0 2 1 0 12 Bma6N 0 0 0 0 0 0 0 Tricine-SDS-PAGE analysis showed that differences between stressed and un-stressed protein production varied greatly between both Lactobacillus and Bifidobacterium genera, and also between each individual LAB (Figure  1). Figure  1 shows the differences in the extra-cellular protein abundance of stressed lactobacilli L. kunkeei Fhon2N and Lactobacillus Fhon13N compared to unstressed controls; there were no differences in bands between stressed and un-stressed controls for the Bifidobacterium Bin7N.